Zeiss LSM 710 NLO
Contact: Tomasz Węgierski
Access: training required
Access fee for academic users: 75 PLN net per hour
Access fee for commercial users: 150 PLN net per hour
General Description
Confocal and 2-photon microscope on inverted stand Zeiss Axio Observer Z1. Lasers: 405 nm, Argon laser (458, 488, 514 nm), HeNe lasers (543, 594, and 633 nm), and MaiTai eHP DeepSee 2-photon laser (690-1040 nm). Detectors: two standard PMTs, one 32-channel spectral detector, and one T-PMT. Lenses: 10×/0.3 (dry), 20×/0.5 (dry), 40×/1.3 (oil), 40×/1.1 (water), 63×/1.3 (water/glycerol), 100×/1.4 (oil). DIC with lenses: 40×/1.1, 40×/1.3 and 63×/1.3. Incubation cage and heating insert with CO2 delivery for live imaging. Software and hardware autofocus. Acquisition software: ZEN 2009. More advanced experiments can be designed with Multi-Time Series macro.
Applications
- Confocal imaging of fluorescently labeled specimens
- 2-photon imaging (best suited is 40×/1.1 UV-VIS-IR lens, W.D. 0.62 mm)
- Live imaging
- FRAP/FRET (best with Argon laser)
- Spectral imaging/dye unmixing
- Co-localization (with two apochromatic lenses: 40×/1.1 and 100×/1.4)
Sample preparation
Samples must be on glass coverslips for imaging with lenses 40× – 100× (e.g. slides, 35-mm petri dishes with glass bottom, 40-mm Warner Instruments chambers with glass coverslips). Dry lenses (especially 10× (W.D. 5.2 mm), possibly also 20× (W.D. 2 mm)) can also be used with samples on thicker glass/plastic dishes. No special requirements for fluorescent dyes.