Differential Scanning Calorimetry (DSC) is a powerful analytical technique for characterizing the thermal stability of the biological macromolecules.

DSC directly measures heat changes that occur in the sample solution during a precise  increase or decrease of the temperature, analyzing a temperature difference between the sample and the reference cells.

By measuring the change of heat capacity (ΔCp) as a function of temperature, DSC allows the direct determination of the variations of enthalpy (ΔH),  entropy (ΔS), and the mid-transition temperature (Tm, when 50% of the molecules are in  unfolded state), associated to each structural transition.