Equipment room (Fifth floor)
Contacts: K. Skowronek, Roman Szczepanowski
Access: collaboration/contracted research, trained users
General description
The FP-8300 is a user friendly spectrofluorometer with a wide range of accessories that are well suited to biological research. The standard automatic band-pass filters obtain spectra without the artificial peaks
due to second order diffracted light. The Auto-Gain and Auto-SCS functions optimize the S/N for samples with large differences in signal intensity and concentration. Temperature control accessories can be used
for thermal melting studies, providing researchers with fluorescence and thermodynamic data for conformational and folding studies. FRET measurements offer insight on binding and folding dynamics. The FP-8300’s automatic polarizers can perform fluorescence anisotropy measurements to provide information regarding fluorophore mobility.
Kinetic and titration measurements can also be performed with the dedicated stopped-flow and autotitrator systems.
Features
- Sophisticated optical system with additional features for biological applications
- High sensitivity S/N > 2,800 (RMS)
- High resolution of 1.0 nm
- Wavelength range: 200 to 750 nm (900 nm optional)