Opera Phenix
Contact: Tomasz Węgierski
Access: training required
Image acquisition service fee for academic users: 100 PLN net per hour
Image acquisition service fee for commercial users: 200 PLN net per hour
Image analysis: ask Tomasz Węgierski
General Description
The Opera Phenix from PerkinElmer is an automated confocal spinning disk microscope. It is equipped with a robotic arm for automatic plate handling, two cameras and four laser lines (405, 488 561, 640 nm). The microscope has four air objectives (1.25×, 5×, 10× and 20×) and two water objectives with automatic immersion system (20× and 40×). The system has software for unbiased image analysis with options of phenotypic screening and machine learning algorithms
Applications
- Confocal imaging of fluorescently labeled multi-well plates
- Bright-field and digital contrast imaging
- Live imaging
- Co-localization
Sample preparation
Samples must be on multi-well plates suitable for microscopy. No special requirements for fluorescent dyes.
Further Reading
- Opera Phenix User Manual
- Opera Phenix Application Guide
- Image Analysis Guide