Covaris M220 Focused-ultrasonicator
Contact: Katarzyna Misztal
Access: short training on request, no reservation needed
Covaris M220 ultrasonicator operates with Adaptive Focused Acoustics (AFA) technology at high frequencies (>400 KHz) to generate fragment sizes between 150 and 5000 bp with highly reproducible distribution and thus standarized results. The process is quiet and isothermal (under continous temperature control) with no risk of heat-damage of the sample. One sample can be sheared per run.
Different tubes are available for DNA/RNA/chromatin shearing.
MicroTUBE -15 or 50 or 500
15 ul or 50 ul or 500 ul
150 – 550 bp
100 – 1500 bp
MiniTUBE clear, blue or red
2 kb, 3 kb or 5kb
Tubes for 50 ul and 130 ul are available in IIMCB Core Facility.
- DNA, RNA or chromatin shearing for e.g Next Generation Sequencing
- Quick Guide: DNA Shearing with M220.pdf
- Quick Guide: Fragmentation of Total RNA with M220.pdf
- Check the covaris webpage for more details.