General Description

The Blue Pippin is an electrophorsis-based, automated size selection tool for wide range of  DNA fragments (100bp – 50kb). With pulsed-field electrophoresis it allows to resolve and collect high molecular weight DNA. BluePippin works with disposible and precasted agarose gel cassettes. Up to 5 samples per gel can be run.

DNA Gel Cassette types and target ranges: 

• 2.0 % agarose, 100 – 600 bp (available in Core Facility)
• 1.5 % agarose, 250 – 1.5 kb
• 0.75 % agarose, 1 – 50 kb (available in Core Facility) 
• 3.0 % agarose, 90 – 200 bp

Applications

• miRNA library isolation
• DNA, RNA and ChIP sequencing
• Long-reads sequencing (PacBio, Oxford Nanopore) 

Further reading

1.  Blue Pippin Operation Manual.pdf
2. Check the Sage Science webpage for more details and manuals.