General Description
Bioruptor Pico is a low frequencies ultrasonicator with an integrated cooling system to generate fragment sizes between 150 and 1000 bp with highly reproducible distribution. It is flexible in volumes (20 ul – 2 ml) and allows shear up to 16 samples simultaneously. Different tube holders are available.
Holders (available in Core Facility) and recommended sample volumes:
- 16 x 0.2 ml tubes, 20 – 100 ul
- 6 x 1.5 ml tubes, 100 – 300 ul
- 6 x 15 ml tubes, 300 ul – 2 ml
Applications
- DNA, RNA and chromatin shearing for e.g Next Generation Sequencing
- Protein extraction from tissues and cells
Further reading
Bioruptor Pico Quickguide
Chromatin Shearing Guide
DNA shearing guide
Which tubes for Bioruptor Pico