{"id":737,"date":"2020-04-27T15:39:53","date_gmt":"2020-04-27T15:39:53","guid":{"rendered":"http:\/\/iimcb.e-kei.pl\/corefacility\/?page_id=737"},"modified":"2026-01-20T17:49:29","modified_gmt":"2026-01-20T17:49:29","slug":"vp-dsc","status":"publish","type":"page","link":"https:\/\/core-facility.iimcb.gov.pl\/vp-dsc\/","title":{"rendered":"VP-DSC"},"content":{"rendered":"\t\t
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VP-DSC MicroCal\/Malvern Microcalorimetry<\/strong><\/span><\/p>\n

Room  612<\/span>
<\/strong><\/span><\/p>\n

Contact: <\/strong><\/span>Roman Szczepanowski<\/a><\/span><\/p>\n

Access to the instrument after short training <\/span><\/strong><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\"VP-DSC\"\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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<\/p>

Differential Scanning Calorimetry (DSC) is a powerful analytical technique for characterizing the thermal stability of the biological macromolecules.<\/span><\/p>

DSC directly measures heat changes that occur in the sample solution during a precise \u00a0<\/span>increase or decrease of the temperature, analyzing a temperature difference between the sample and the reference cells.<\/span><\/p>

By measuring the change of heat capacity (\u0394Cp) as a function of temperature, DSC allows the direct determination of the variations of enthalpy (\u0394H),\u00a0 entropy (\u0394S), and the mid-transition temperature (Tm, when 50% of the molecules are in\u00a0 <\/span>unfolded state), associated to each structural transition.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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<\/p>

Operating Temperature Range:\u00a0 <\/span>-10\u00b0 to 130\u00b0C<\/span><\/p>

Cell Design: Tantalum, coin shape, 0.5 ml. For safe loading min. 1.2 ml of\u00a0 solution is necessary.<\/span><\/p>

Up-scans speed: 0\u00b0C\/hr to 90\u00b0C\/hr, down-scans: scan rates:\u00a0 <\/span>0\u00b0C\/hr to -60\u00b0C\/hr<\/span><\/p>

Further reading<\/span><\/strong><\/span><\/span><\/p>

VP-DSC User’s Manual<\/a><\/p>

\u00a0<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"

VP-DSC MicroCal\/Malvern Microcalorimetry Room  612 Contact: Roman Szczepanowski Access to the instrument after short training Differential Scanning Calorimetry (DSC) is a powerful analytical technique for characterizing the thermal stability of the biological macromolecules. DSC directly measures heat changes that occur in the sample solution during a precise \u00a0increase or decrease of the temperature, analyzing a […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"elementor_header_footer","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"class_list":["post-737","page","type-page","status-publish","hentry","entry"],"_links":{"self":[{"href":"https:\/\/core-facility.iimcb.gov.pl\/wp-json\/wp\/v2\/pages\/737","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/core-facility.iimcb.gov.pl\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/core-facility.iimcb.gov.pl\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/core-facility.iimcb.gov.pl\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/core-facility.iimcb.gov.pl\/wp-json\/wp\/v2\/comments?post=737"}],"version-history":[{"count":13,"href":"https:\/\/core-facility.iimcb.gov.pl\/wp-json\/wp\/v2\/pages\/737\/revisions"}],"predecessor-version":[{"id":2793,"href":"https:\/\/core-facility.iimcb.gov.pl\/wp-json\/wp\/v2\/pages\/737\/revisions\/2793"}],"wp:attachment":[{"href":"https:\/\/core-facility.iimcb.gov.pl\/wp-json\/wp\/v2\/media?parent=737"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}