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\/>  <w:LsdException Locked=\"false\" Priority=\"69\" Name=\"Medium Grid 3\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"70\" Name=\"Dark List\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"71\" Name=\"Colorful Shading\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"72\" Name=\"Colorful List\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"73\" Name=\"Colorful Grid\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"60\" Name=\"Light Shading Accent 1\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"61\" Name=\"Light List Accent 1\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"62\" Name=\"Light Grid Accent 1\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"63\" Name=\"Medium Shading 1 Accent 1\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"64\" Name=\"Medium Shading 2 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Locked=\"false\" Priority=\"60\" Name=\"Light Shading Accent 3\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"61\" Name=\"Light List Accent 3\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"62\" Name=\"Light Grid Accent 3\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"63\" Name=\"Medium Shading 1 Accent 3\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"64\" Name=\"Medium Shading 2 Accent 3\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"65\" Name=\"Medium List 1 Accent 3\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"66\" Name=\"Medium List 2 Accent 3\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"67\" Name=\"Medium Grid 1 Accent 3\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"68\" Name=\"Medium Grid 2 Accent 3\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"69\" Name=\"Medium Grid 3 Accent 3\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"70\" Name=\"Dark List Accent 3\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"71\" Name=\"Colorful Shading Accent 3\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"72\" Name=\"Colorful List Accent 3\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"73\" Name=\"Colorful Grid Accent 3\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"60\" Name=\"Light Shading Accent 4\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"61\" Name=\"Light List Accent 4\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"62\" Name=\"Light Grid Accent 4\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"63\" Name=\"Medium Shading 1 Accent 4\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"64\" Name=\"Medium 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2\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"51\" Name=\"List Table 6 Colorful Accent 2\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"52\" Name=\"List Table 7 Colorful Accent 2\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"46\" Name=\"List Table 1 Light Accent 3\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"47\" Name=\"List Table 2 Accent 3\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"48\" Name=\"List Table 3 Accent 3\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"49\" Name=\"List Table 4 Accent 3\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"50\" Name=\"List Table 5 Dark Accent 3\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"51\" Name=\"List Table 6 Colorful Accent 3\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"52\" Name=\"List Table 7 Colorful Accent 3\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"46\" Name=\"List Table 1 Light Accent 4\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"47\" Name=\"List Table 2 Accent 4\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"48\" Name=\"List Table 3 Accent 4\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"49\" Name=\"List Table 4 Accent 4\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"50\" Name=\"List Table 5 Dark Accent 4\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"51\" Name=\"List Table 6 Colorful Accent 4\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"52\" Name=\"List Table 7 Colorful Accent 4\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"46\" Name=\"List Table 1 Light Accent 5\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"47\" Name=\"List Table 2 Accent 5\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"48\" Name=\"List Table 3 Accent 5\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"49\" Name=\"List Table 4 Accent 5\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"50\" Name=\"List Table 5 Dark Accent 5\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"51\" Name=\"List Table 6 Colorful Accent 5\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"52\" Name=\"List Table 7 Colorful Accent 5\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"46\" Name=\"List Table 1 Light Accent 6\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"47\" Name=\"List Table 2 Accent 6\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"48\" Name=\"List Table 3 Accent 6\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"49\" Name=\"List Table 4 Accent 6\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"50\" Name=\"List Table 5 Dark Accent 6\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"51\" Name=\"List Table 6 Colorful Accent 6\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"52\" Name=\"List Table 7 Colorful Accent 6\"><\/w:LsdException><br \/> <\/w:LatentStyles><br \/><\/xml><![endif]--><!-- [if gte mso 10]><\/p>\n<style>\n \/* Style Definitions *\/<br \/> table.MsoNormalTable<br \/>\t{mso-style-name:\"Table Normal\";<br \/>\tmso-tstyle-rowband-size:0;<br \/>\tmso-tstyle-colband-size:0;<br \/>\tmso-style-noshow:yes;<br \/>\tmso-style-priority:99;<br \/>\tmso-style-parent:\"\";<br \/>\tmso-padding-alt:0cm 5.4pt 0cm 5.4pt;<br \/>\tmso-para-margin-top:0cm;<br \/>\tmso-para-margin-right:0cm;<br \/>\tmso-para-margin-bottom:8.0pt;<br \/>\tmso-para-margin-left:0cm;<br \/>\tline-height:107%;<br \/>\tmso-pagination:widow-orphan;<br \/>\tfont-size:11.0pt;<br \/>\tfont-family:\"Calibri\",sans-serif;<br \/>\tmso-ascii-font-family:Calibri;<br \/>\tmso-ascii-theme-font:minor-latin;<br \/>\tmso-hansi-font-family:Calibri;<br \/>\tmso-hansi-theme-font:minor-latin;<br \/>\tmso-bidi-font-family:\"Times New Roman\";<br \/>\tmso-bidi-theme-font:minor-bidi;<br \/>\tmso-ansi-language:PL;}<br \/><\/style>\n<p><![endif]--><\/p><p style=\"text-align: center;\" align=\"center\"><span style=\"font-size: 14pt; color: #000000;\"><b style=\"mso-bidi-font-weight: normal;\"><span style=\"line-height: 107%;\">SEC-MALS<\/span><\/b><\/span><\/p><p style=\"text-align: center;\" align=\"center\"><span style=\"font-size: 14pt; color: #000000;\"><b style=\"mso-bidi-font-weight: normal;\"><span style=\"line-height: 107%;\">Size Exclusion Chromatography \u2013 Multi-Angle Light Scattering<\/span><\/b><\/span><\/p><p style=\"text-align: center;\"><span style=\"font-family: georgia, palatino, serif; font-size: 14pt; color: #000000;\"><b style=\"mso-bidi-font-weight: normal;\">Room 607A<\/b><\/span><\/p><p style=\"margin-bottom: 2.0pt; text-align: center; line-height: normal;\" align=\"center\"><span style=\"font-family: georgia, palatino, serif; font-size: 14pt;\"><b style=\"mso-bidi-font-weight: normal;\"><span style=\"color: #000000;\">Contact:<\/span> <a href=\"https:\/\/core-facility.iimcb.gov.pl\/krzysztof-skowronek\/\">Krzysztof Skowronek<\/a>, <a href=\"https:\/\/core-facility.iimcb.gov.pl\/matylda\/\">Matylda Wac\u0142awska<\/a><\/b><\/span><\/p><p style=\"margin-bottom: 2.0pt; text-align: center; line-height: normal;\" align=\"center\"><span style=\"font-family: georgia, palatino, serif; font-size: 14pt; color: #000000;\"><b style=\"mso-bidi-font-weight: normal;\">Access: collaboration\/contracted research, experienced users<\/b><\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-3186f81 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"3186f81\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-63a1d78\" data-id=\"63a1d78\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-b946ba1 elementor-widget elementor-widget-image\" data-id=\"b946ba1\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"473\" height=\"278\" src=\"https:\/\/core-facility.iimcb.gov.pl\/wp-content\/uploads\/2020\/04\/MALS1.jpg\" class=\"attachment-large size-large wp-image-447\" alt=\"MALS\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-8f82410 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"8f82410\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-bb02d5f\" data-id=\"bb02d5f\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-ac213ef elementor-widget elementor-widget-text-editor\" data-id=\"ac213ef\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p class=\"MsoNormal\" style=\"margin-bottom: 2.0pt; text-align: justify; line-height: normal;\"><span style=\"font-family: georgia, palatino, serif; font-size: 12pt;\"><b style=\"mso-bidi-font-weight: normal;\">\u00a0<\/b><\/span><span style=\"font-family: georgia, palatino, serif; font-size: 14pt; color: #339966;\"><b style=\"mso-bidi-font-weight: normal;\">Components:<\/b><\/span><\/p><ul><li class=\"MsoListParagraphCxSpFirst\" style=\"text-align: justify; text-indent: -18pt; line-height: normal;\"><span style=\"font-family: georgia, palatino, serif; font-size: 12pt; color: #000000;\"><span style=\"mso-list: Ignore;\"><span style=\"font-style: normal; font-variant: normal; font-stretch: normal; line-height: normal; font-size-adjust: none; font-kerning: auto; font-optical-sizing: auto; font-language-override: normal; font-feature-settings: normal; font-variation-settings: normal;\">\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 <\/span><\/span>Binary HPLC Pump 1525 (Waters)<\/span><\/li><li class=\"MsoListParagraphCxSpMiddle\" style=\"text-align: justify; text-indent: -18pt; line-height: normal;\"><span style=\"font-family: georgia, palatino, serif; font-size: 12pt; color: #000000;\"><span style=\"mso-list: Ignore;\"><span style=\"font-style: normal; font-variant: normal; font-stretch: normal; line-height: normal; font-size-adjust: none; font-kerning: auto; font-optical-sizing: auto; font-language-override: normal; font-feature-settings: normal; font-variation-settings: normal;\">\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 <\/span><\/span>Inline Prep Degasser (Waters)<\/span><\/li><li class=\"MsoListParagraphCxSpMiddle\" style=\"text-align: justify; text-indent: -18pt; line-height: normal;\"><span style=\"font-family: georgia, palatino, serif; font-size: 12pt; color: #000000;\"><span style=\"mso-list: Ignore;\"><span style=\"font-style: normal; font-variant: normal; font-stretch: normal; line-height: normal; font-size-adjust: none; font-kerning: auto; font-optical-sizing: auto; font-language-override: normal; font-feature-settings: normal; font-variation-settings: normal;\">\u00a0\u00a0\u00a0\u00a0 \u00a0\u00a0\u00a0\u00a0 <\/span><\/span>Manual Injection module Flexinject (100 \u00b5l and 5 ml loops) (Waters)<\/span><\/li><li class=\"MsoListParagraphCxSpMiddle\" style=\"text-align: justify; text-indent: -18pt; line-height: normal;\"><span style=\"font-family: georgia, palatino, serif; font-size: 12pt; color: #000000;\"><span style=\"mso-list: Ignore;\"><span style=\"font-style: normal; font-variant: normal; font-stretch: normal; line-height: normal; font-size-adjust: none; font-kerning: auto; font-optical-sizing: auto; font-language-override: normal; font-feature-settings: normal; font-variation-settings: normal;\">\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 <\/span><\/span>UV\/VIS Detector 2489 (Waters)<\/span><\/li><li class=\"MsoListParagraphCxSpMiddle\" style=\"text-align: justify; text-indent: -18pt; line-height: normal;\"><span style=\"font-family: georgia, palatino, serif; font-size: 12pt; color: #000000;\"><span style=\"mso-list: Ignore;\"><span style=\"font-style: normal; font-variant: normal; font-stretch: normal; line-height: normal; font-size-adjust: none; font-kerning: auto; font-optical-sizing: auto; font-language-override: normal; font-feature-settings: normal; font-variation-settings: normal;\">\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 <\/span><\/span>Column oven (for up to 30 cm columns) (Waters)<\/span><\/li><li class=\"MsoListParagraphCxSpMiddle\" style=\"text-align: justify; text-indent: -18pt; line-height: normal;\"><span style=\"font-family: georgia, palatino, serif; font-size: 12pt; color: #000000;\"><span style=\"mso-list: Ignore;\"><span style=\"font-style: normal; font-variant: normal; font-stretch: normal; line-height: normal; font-size-adjust: none; font-kerning: auto; font-optical-sizing: auto; font-language-override: normal; font-feature-settings: normal; font-variation-settings: normal;\">\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 <\/span><\/span>Dawn 8+, 8 angles MALS detector (Wyatt)<\/span><\/li><li class=\"MsoListParagraphCxSpMiddle\" style=\"text-align: justify; text-indent: -18pt; line-height: normal;\"><span style=\"font-family: georgia, palatino, serif; font-size: 12pt; color: #000000;\"><span style=\"mso-list: Ignore;\"><span style=\"font-style: normal; font-variant: normal; font-stretch: normal; line-height: normal; font-size-adjust: none; font-kerning: auto; font-optical-sizing: auto; font-language-override: normal; font-feature-settings: normal; font-variation-settings: normal;\">\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 <\/span><\/span>Optilab T-rEX refractive index detector (Wyatt)<\/span><\/li><li class=\"MsoListParagraphCxSpLast\" style=\"text-align: justify; text-indent: -18pt; line-height: normal;\"><span style=\"font-family: georgia, palatino, serif; font-size: 12pt; color: #000000;\"><span style=\"mso-list: Ignore;\"><span style=\"font-style: normal; font-variant: normal; font-stretch: normal; line-height: normal; font-size-adjust: none; font-kerning: auto; font-optical-sizing: auto; font-language-override: normal; font-feature-settings: normal; font-variation-settings: normal;\">\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 <\/span><\/span>Fraction Collector III (Waters)<\/span><\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-29552fa elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"29552fa\" data-element_type=\"section\" 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4\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"72\" Name=\"Colorful List Accent 4\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"73\" Name=\"Colorful Grid Accent 4\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"60\" Name=\"Light Shading Accent 5\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"61\" Name=\"Light List Accent 5\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"62\" Name=\"Light Grid Accent 5\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"63\" Name=\"Medium Shading 1 Accent 5\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"64\" Name=\"Medium Shading 2 Accent 5\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"65\" Name=\"Medium List 1 Accent 5\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"66\" Name=\"Medium List 2 Accent 5\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"67\" Name=\"Medium Grid 1 Accent 5\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"68\" Name=\"Medium Grid 2 Accent 5\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"69\" Name=\"Medium Grid 3 Accent 5\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"70\" Name=\"Dark List Accent 5\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"71\" Name=\"Colorful Shading Accent 5\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"72\" Name=\"Colorful List Accent 5\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"73\" Name=\"Colorful Grid Accent 5\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"60\" Name=\"Light Shading Accent 6\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"61\" Name=\"Light List Accent 6\"><\/w:LsdException><br \/>  <w:LsdException 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Name=\"Bibliography\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"39\" SemiHidden=\"true\" UnhideWhenUsed=\"true\" QFormat=\"true\" Name=\"TOC Heading\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"41\" Name=\"Plain Table 1\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"42\" Name=\"Plain Table 2\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"43\" Name=\"Plain Table 3\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"44\" Name=\"Plain Table 4\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"45\" Name=\"Plain Table 5\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"40\" Name=\"Grid Table Light\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"46\" Name=\"Grid Table 1 Light\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"47\" Name=\"Grid Table 2\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"48\" Name=\"Grid Table 3\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"49\" Name=\"Grid Table 4\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"50\" Name=\"Grid Table 5 Dark\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"51\" Name=\"Grid Table 6 Colorful\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"52\" Name=\"Grid Table 7 Colorful\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"46\" Name=\"Grid Table 1 Light Accent 1\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"47\" Name=\"Grid Table 2 Accent 1\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"48\" Name=\"Grid Table 3 Accent 1\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"49\" Name=\"Grid Table 4 Accent 1\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"50\" Name=\"Grid Table 5 Dark Accent 1\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"51\" Name=\"Grid Table 6 Colorful Accent 1\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"52\" Name=\"Grid Table 7 Colorful Accent 1\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"46\" Name=\"Grid Table 1 Light Accent 2\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"47\" Name=\"Grid Table 2 Accent 2\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"48\" Name=\"Grid Table 3 Accent 2\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"49\" Name=\"Grid Table 4 Accent 2\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"50\" Name=\"Grid Table 5 Dark Accent 2\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"51\" Name=\"Grid Table 6 Colorful Accent 2\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"52\" Name=\"Grid Table 7 Colorful Accent 2\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"46\" Name=\"Grid Table 1 Light Accent 3\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"47\" Name=\"Grid Table 2 Accent 3\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"48\" Name=\"Grid Table 3 Accent 3\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"49\" Name=\"Grid Table 4 Accent 3\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"50\" Name=\"Grid Table 5 Dark Accent 3\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"51\" Name=\"Grid Table 6 Colorful Accent 3\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"52\" Name=\"Grid Table 7 Colorful Accent 3\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"46\" Name=\"Grid Table 1 Light Accent 4\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"47\" Name=\"Grid Table 2 Accent 4\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"48\" Name=\"Grid Table 3 Accent 4\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"49\" Name=\"Grid Table 4 Accent 4\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"50\" Name=\"Grid Table 5 Dark Accent 4\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"51\" Name=\"Grid Table 6 Colorful Accent 4\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"52\" Name=\"Grid Table 7 Colorful Accent 4\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"46\" Name=\"Grid Table 1 Light Accent 5\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"47\" Name=\"Grid Table 2 Accent 5\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"48\" Name=\"Grid Table 3 Accent 5\"><\/w:LsdException><br \/>  <w:LsdException 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Locked=\"false\" Priority=\"51\" Name=\"Grid Table 6 Colorful Accent 6\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"52\" Name=\"Grid Table 7 Colorful Accent 6\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"46\" Name=\"List Table 1 Light\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"47\" Name=\"List Table 2\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"48\" Name=\"List Table 3\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"49\" Name=\"List Table 4\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"50\" Name=\"List Table 5 Dark\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"51\" Name=\"List Table 6 Colorful\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"52\" Name=\"List Table 7 Colorful\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"46\" Name=\"List Table 1 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Table 2 Accent 6\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"48\" Name=\"List Table 3 Accent 6\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"49\" Name=\"List Table 4 Accent 6\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"50\" Name=\"List Table 5 Dark Accent 6\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"51\" Name=\"List Table 6 Colorful Accent 6\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"52\" Name=\"List Table 7 Colorful Accent 6\"><\/w:LsdException><br \/> <\/w:LatentStyles><br \/><\/xml><![endif]--><!-- [if gte mso 10]><\/p>\n<style>\n \/* Style Definitions *\/<br \/> table.MsoNormalTable<br \/>\t{mso-style-name:\"Table Normal\";<br \/>\tmso-tstyle-rowband-size:0;<br \/>\tmso-tstyle-colband-size:0;<br \/>\tmso-style-noshow:yes;<br \/>\tmso-style-priority:99;<br \/>\tmso-style-parent:\"\";<br \/>\tmso-padding-alt:0cm 5.4pt 0cm 5.4pt;<br \/>\tmso-para-margin-top:0cm;<br \/>\tmso-para-margin-right:0cm;<br \/>\tmso-para-margin-bottom:8.0pt;<br \/>\tmso-para-margin-left:0cm;<br \/>\tline-height:107%;<br \/>\tmso-pagination:widow-orphan;<br \/>\tfont-size:11.0pt;<br \/>\tfont-family:\"Calibri\",sans-serif;<br \/>\tmso-ascii-font-family:Calibri;<br \/>\tmso-ascii-theme-font:minor-latin;<br \/>\tmso-hansi-font-family:Calibri;<br \/>\tmso-hansi-theme-font:minor-latin;<br \/>\tmso-bidi-font-family:\"Times New Roman\";<br \/>\tmso-bidi-theme-font:minor-bidi;<br \/>\tmso-ansi-language:PL;}<br \/><\/style>\n<p><![endif]--><\/p><p class=\"MsoNormal\" style=\"margin-bottom: 2.0pt; text-align: justify; line-height: normal;\"><span style=\"color: #339966; font-family: georgia, palatino, serif; font-size: 14pt;\"><b style=\"mso-bidi-font-weight: normal;\">General description<\/b><\/span><\/p><p class=\"MsoNormal\" style=\"margin-bottom: 2.0pt; text-align: justify; line-height: normal;\"><span style=\"font-size: 12pt; font-family: georgia, palatino, serif; color: #000000;\">This instrument allows calibration-independent measurement of molecular mass in each fraction of SEC. UV\/VIS, MALS and refractive index (RI) detectors are in-line with outflow from HPLC system. UV\/VIS and RI detectors are used for independent concentration quantification while signal from 8 MALS detectors provides information on light scattering at different angles. MALS data are analysed by selected mathematical formula (by default it is Zimm eqution) to obtain calibration-independent time-resolved molecular mass measurement. <br \/><\/span><\/p><p class=\"MsoNormal\" style=\"margin-bottom: 2.0pt; text-align: justify; line-height: normal;\"><span style=\"font-size: 12pt; font-family: georgia, palatino, serif; color: #000000;\"><b style=\"mso-bidi-font-weight: normal;\">NOTE<\/b>: In contrast to analytical ultracentrifugation[L] MALS directly measures radius of gyration (not hydrodynamic radius). SEC fractions can be collected by fraction collector for further analysis. Currently we offer two SEC columns:<\/span><\/p><ul><li><span style=\"font-family: georgia, palatino, serif; font-size: 12pt; color: #000000;\">BioSuite 450 \u00c5 (8 \u00b5m, 7.8 x 300 mm) with resolution range 20 kDa \u2013 1 MDa (for globular proteins)<\/span><\/li><li><span style=\"font-family: georgia, palatino, serif; font-size: 12pt; color: #000000;\">XBridge Protein BEH SEC 200 \u00c5 (3.5 \u00b5m, 7.8 x 300 mm) with resolution range 10 kDa \u2013 450 kDa (for globular proteins)<\/span><\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-445fe42 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"445fe42\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div 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Locked=\"false\" Priority=\"70\" Name=\"Dark List\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"71\" Name=\"Colorful Shading\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"72\" Name=\"Colorful List\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"73\" Name=\"Colorful Grid\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"60\" Name=\"Light Shading Accent 1\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"61\" Name=\"Light List Accent 1\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"62\" Name=\"Light Grid Accent 1\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"63\" Name=\"Medium Shading 1 Accent 1\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"64\" Name=\"Medium Shading 2 Accent 1\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"65\" Name=\"Medium List 1 Accent 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6\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"51\" Name=\"List Table 6 Colorful Accent 6\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"52\" Name=\"List Table 7 Colorful Accent 6\"><\/w:LsdException><br \/> <\/w:LatentStyles><br \/><\/xml><![endif]--><!-- [if gte mso 10]><\/p>\n<style>\n \/* Style Definitions *\/<br \/> table.MsoNormalTable<br \/>\t{mso-style-name:\"Table Normal\";<br \/>\tmso-tstyle-rowband-size:0;<br \/>\tmso-tstyle-colband-size:0;<br \/>\tmso-style-noshow:yes;<br \/>\tmso-style-priority:99;<br \/>\tmso-style-parent:\"\";<br \/>\tmso-padding-alt:0cm 5.4pt 0cm 5.4pt;<br \/>\tmso-para-margin-top:0cm;<br \/>\tmso-para-margin-right:0cm;<br \/>\tmso-para-margin-bottom:8.0pt;<br \/>\tmso-para-margin-left:0cm;<br \/>\tline-height:107%;<br \/>\tmso-pagination:widow-orphan;<br \/>\tfont-size:11.0pt;<br \/>\tfont-family:\"Calibri\",sans-serif;<br \/>\tmso-ascii-font-family:Calibri;<br \/>\tmso-ascii-theme-font:minor-latin;<br \/>\tmso-hansi-font-family:Calibri;<br \/>\tmso-hansi-theme-font:minor-latin;<br \/>\tmso-bidi-font-family:\"Times New Roman\";<br \/>\tmso-bidi-theme-font:minor-bidi;<br \/>\tmso-ansi-language:PL;}<br \/><\/style>\n<p><![endif]--><\/p><p class=\"MsoNormal\" style=\"margin-bottom: 2.0pt; text-align: justify; line-height: normal;\"><em><span style=\"font-size: 10.0pt; mso-ansi-language: EN-US;\">SEC-MALS of Waters molecular weight standards resolved on BioSuite 450 column. Peaks: 1 \u2013 dimeric thyroglobulin (1.4 MDa), 2 \u2013 monomeric thyroglobulin (660 kDa), 3 \u2013 IgG (150 kDa), 4 \u2013 BSA (66.4 kDa)<\/span><\/em><\/p><p class=\"MsoNormal\" style=\"margin-bottom: 2.0pt; text-align: justify; line-height: normal;\"><b style=\"mso-bidi-font-weight: normal;\"><span style=\"font-size: 14.0pt; color: #0070c0; mso-ansi-language: EN-US;\">\u00a0<\/span><\/b><\/p><p class=\"MsoNormal\" style=\"margin-bottom: 2.0pt; text-align: justify; line-height: normal;\"><span style=\"color: #008000; font-family: georgia, palatino, serif; font-size: 14pt;\"><b style=\"mso-bidi-font-weight: normal;\">Applications<\/b><\/span><\/p><ul><li><span style=\"font-family: georgia, palatino, serif; font-size: 12pt; color: #000000;\"><span style=\"mso-list: Ignore;\"><span style=\"font-style: normal; font-variant: normal; font-weight: normal; font-stretch: normal; line-height: normal; font-size-adjust: none; font-kerning: auto; font-optical-sizing: auto; font-language-override: normal; font-feature-settings: normal; font-variation-settings: normal;\">\u00a0<\/span><\/span>Measurement of molecular mass of various biological macromolecules (polysaccharides [using RI detector], proteins, nucleic acids and their complexes)<\/span><\/li><li><span style=\"font-size: 12pt; font-family: georgia, palatino, serif; color: #000000;\">Estimating homogeneity of macromolecule preparation<\/span><\/li><li><span style=\"font-size: 12pt; font-family: georgia, palatino, serif; color: #000000;\">Estimating monodispersity of macromolecules in each fraction<\/span><\/li><li><span style=\"font-size: 12pt; font-family: georgia, palatino, serif; color: #000000;\">Assisting in the last step of macromolecular complex purification (using fraction collector)<\/span><\/li><li><span style=\"font-size: 12pt; font-family: georgia, palatino, serif; color: #000000;\">Determination of stoichiometry of macromolecular complexes<\/span><\/li><\/ul><p class=\"MsoNormal\" style=\"margin-bottom: 2.0pt; text-align: justify; line-height: normal;\"><span style=\"color: #008000; font-family: georgia, palatino, serif; font-size: 14pt;\"><b style=\"mso-bidi-font-weight: normal;\">Sample requirements<\/b><\/span><\/p><p class=\"MsoNormal\" style=\"margin-bottom: 2.0pt; text-align: justify; line-height: normal;\"><span style=\"font-family: georgia, palatino, serif; font-size: 12pt; color: #000000;\">At least 120 \u00b5l at concentration adequate for proper estimation of concentration from relative RI (at least 1-2 mg\/ml) equilibrated in chromatography buffer. Sample should be filtered through 0.2 \u00b5m filter.<\/span><\/p><p class=\"MsoNormal\" style=\"margin-bottom: 2.0pt; text-align: justify; line-height: normal;\"><span style=\"font-size: 12pt; font-family: georgia, palatino, serif;\">\u00a0<\/span><\/p><p class=\"MsoNormal\" style=\"margin-bottom: 2.0pt; text-align: justify; line-height: normal;\"><span style=\"color: #339966; font-family: georgia, palatino, serif; font-size: 14pt;\"><b style=\"mso-bidi-font-weight: normal;\">Further reading<\/b><\/span><\/p><p class=\"MsoListParagraphCxSpFirst\" style=\"margin-bottom: 2.0pt; mso-add-space: auto; text-align: justify; text-indent: -18.0pt; line-height: normal; mso-list: l3 level1 lfo4;\"><span style=\"font-family: georgia, palatino, serif; font-size: 12pt;\"><span style=\"mso-list: Ignore;\">1.<span style=\"font-style: normal; font-variant: normal; font-weight: normal; font-stretch: normal; line-height: normal; font-size-adjust: none; font-kerning: auto; font-optical-sizing: auto; font-language-override: normal; font-feature-settings: normal; font-variation-settings: normal;\">\u00a0\u00a0\u00a0\u00a0\u00a0 <\/span><\/span><span class=\"MsoHyperlink\"><a href=\"https:\/\/www.wyatt.com\/library\/theory\/multi-angle-light-scattering-theory.html\">https:\/\/www.wyatt.com\/library\/theory\/multi-angle-light-scattering-theory.html<\/a><\/span><\/span><\/p><p class=\"MsoListParagraphCxSpLast\" style=\"margin-bottom: 2.0pt; mso-add-space: auto; text-align: justify; text-indent: -18.0pt; line-height: normal; mso-list: l3 level1 lfo4;\"><span style=\"font-family: georgia, palatino, serif; font-size: 12pt;\"><span style=\"mso-list: Ignore;\">2. <\/span><span style=\"color: #000000;\">Mogridge J. Using light scattering to determine the stoichiometry of protein complexes. Methods Mol Biol. 2015;1278:233-8. doi: 10.1007\/978-1-4939-2425-7_14. PubMed PMID: 25859953.<\/span><\/span><\/p><p class=\"MsoNormal\" style=\"text-align: justify; line-height: normal; margin: 0cm 0cm 2.0pt 18.0pt;\"><span style=\"font-size: 12pt; font-family: georgia, palatino, serif;\">\u00a0<\/span><\/p><p class=\"MsoNormal\" style=\"margin-bottom: 2.0pt; text-align: justify; line-height: normal;\"><span style=\"font-size: 12pt; font-family: georgia, palatino, serif;\">\u00a0<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>SEC-MALS Size Exclusion Chromatography \u2013 Multi-Angle Light Scattering Room 607A Contact: Krzysztof Skowronek, Matylda Wac\u0142awska Access: collaboration\/contracted research, experienced users \u00a0Components: \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Binary HPLC Pump 1525 (Waters) \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Inline Prep Degasser (Waters) \u00a0\u00a0\u00a0\u00a0 \u00a0\u00a0\u00a0\u00a0 Manual Injection module Flexinject (100 \u00b5l and 5 ml loops) (Waters) \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 UV\/VIS Detector 2489 (Waters) \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Column oven (for up [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"elementor_header_footer","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"class_list":["post-445","page","type-page","status-publish","hentry","entry"],"_links":{"self":[{"href":"https:\/\/core-facility.iimcb.gov.pl\/wp-json\/wp\/v2\/pages\/445","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/core-facility.iimcb.gov.pl\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/core-facility.iimcb.gov.pl\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/core-facility.iimcb.gov.pl\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/core-facility.iimcb.gov.pl\/wp-json\/wp\/v2\/comments?post=445"}],"version-history":[{"count":19,"href":"https:\/\/core-facility.iimcb.gov.pl\/wp-json\/wp\/v2\/pages\/445\/revisions"}],"predecessor-version":[{"id":2767,"href":"https:\/\/core-facility.iimcb.gov.pl\/wp-json\/wp\/v2\/pages\/445\/revisions\/2767"}],"wp:attachment":[{"href":"https:\/\/core-facility.iimcb.gov.pl\/wp-json\/wp\/v2\/media?parent=445"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}