{"id":2455,"date":"2025-04-27T18:29:20","date_gmt":"2025-04-27T18:29:20","guid":{"rendered":"https:\/\/core-facility.iimcb.gov.pl\/?page_id=2455"},"modified":"2025-04-28T11:07:36","modified_gmt":"2025-04-28T11:07:36","slug":"mass_photometry","status":"publish","type":"page","link":"https:\/\/core-facility.iimcb.gov.pl\/mass_photometry\/","title":{"rendered":"Mass Photometry"},"content":{"rendered":"\t\t
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Mass Photometry<\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Refeyn TwoMP mass photometer with MassFluidix\u00ae HC system<\/h1>

Room 6B (Sixth floor<\/em>)<\/strong><\/span>
Contacts: Krzyszof Skowronek<\/a>, Roman Szczepanowski<\/a><\/strong><\/span>
Collaboration\/contracted research<\/strong>. Access to instrument\u00a0 <\/span>only by operator or authorized persons.<\/b><\/span><\/p>

\u00a0<\/p>

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Refeyn TwoMP mass photometer<\/h1><\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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<\/p>

Mass<\/b> photometry<\/b> is a revolutionary new method of analyzing molecules. It enables the accurate mass measurements of single molecules in solution, in their native state and without the need for labels. For molecular mass measurements with unmatched sensitivity, speed and simplicity of use, a TwoMP mass photometer offers wide mass range and single-molecule resolution.<\/span><\/p>

  • High-fidelity measurements of molecular mass<\/span><\/p><\/li>

  • Minimal quantities of sample required<\/span><\/p><\/li>

  • Intuitive acquisition and data analysis software\u00a0<\/span><\/p><\/li>

  • Easy setup \u2013 a compact, benchtop instrument with minimum installation requirements<\/span><\/p><\/li><\/ul><\/div><\/div><\/div>

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    Protein-protein interactions<\/span><\/h3><\/div><\/div>

    Antigen-antibody interactions are a prime example of molecular systems that can be studied using mass photometry, which can be applied to determine binding affinities for mono- and multivalent interactions.<\/p>

    Figure 1 illustrates how mass photometry can be
    used to quantify molecular interactions. By measuring the mass of the antibody trastuzumab and its target antigen, Her2, individually and in mixtures, the interactions between individual antibody molecules and target antigens could be quantified.<\/span><\/h6><\/div><\/div><\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t
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    DNA-protein interactions<\/span><\/h3><\/div><\/div>

    Mass photometry can be used to characterise DNA-protein complexes, which are crucial in gene expression, replication and DNA repair.<\/p>

    In this example, mass photometry not only allows detection of DNA binding but also provides information on how the oligomeric state of the protein changes upon DNA binding (Figure 2).<\/i><\/p><\/div><\/div><\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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    Protein oligomerization<\/span><\/h3><\/div><\/div>

    Many proteins adopt a particular oligomeric form under certain conditions. In Figure 1, mass photometry characterises four different proteins: protein A, beta-amylase, urease and thyroglobulin. Through this characterisation, these proteins showcase a range of behaviours \u2013 from purely monomeric (as in Protein A) to a dynamic equilibrium between multiple states.<\/p><\/div><\/div><\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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    Complex formation<\/span><\/h3><\/div><\/div>

    Ribosomes are macromolecular assemblies of protein and RNA, and are central sites for protein synthesis. Bacterial ribosomes (in this example from E. coli) are >2 MDa in size. Magnesium ions play a subtle yet important role in the assembly of intact (70S) complexes. In the absence of magnesium, E. coli ribosomes rapidly disassemble into their 30S and 50S subunits. Mass photometry allows us to monitor these processes (Figure).<\/p><\/div><\/div><\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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    MassFluidix\u00ae HC system<\/h1><\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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    Refeyn\u2019s\n microfluidics system, MassFluidix HC, significantly expands the range \nof sample concentrations amenable to investigation by mass photometry, \nby raising the upper sample concentration limit from the nanomolar to \nthe micromolar range. This enables applications such as the \ncharacterization of low-affinity interactions. <\/span><\/span><\/p>

    <\/span><\/span>MassFluidix\n HC is an add-on for Refeyn\u2019s TwoMP mass photometer and includes a \ncentral unit with fluid controls and a rapid dilution chip.<\/span><\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>


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    Mass Photometry Refeyn TwoMP mass photometer with MassFluidix\u00ae HC system Room 6B (Sixth floor)Contacts: Krzyszof Skowronek, Roman SzczepanowskiCollaboration\/contracted research. Access to instrument\u00a0 only by operator or authorized persons. \u00a0 Refeyn TwoMP mass photometer Mass photometry is a revolutionary new method of analyzing molecules. It enables the accurate mass measurements of single molecules in solution, in […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"elementor_header_footer","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"class_list":["post-2455","page","type-page","status-publish","hentry","entry"],"_links":{"self":[{"href":"https:\/\/core-facility.iimcb.gov.pl\/wp-json\/wp\/v2\/pages\/2455","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/core-facility.iimcb.gov.pl\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/core-facility.iimcb.gov.pl\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/core-facility.iimcb.gov.pl\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/core-facility.iimcb.gov.pl\/wp-json\/wp\/v2\/comments?post=2455"}],"version-history":[{"count":44,"href":"https:\/\/core-facility.iimcb.gov.pl\/wp-json\/wp\/v2\/pages\/2455\/revisions"}],"predecessor-version":[{"id":2598,"href":"https:\/\/core-facility.iimcb.gov.pl\/wp-json\/wp\/v2\/pages\/2455\/revisions\/2598"}],"wp:attachment":[{"href":"https:\/\/core-facility.iimcb.gov.pl\/wp-json\/wp\/v2\/media?parent=2455"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}