{"id":225,"date":"2020-04-13T21:33:17","date_gmt":"2020-04-13T21:33:17","guid":{"rendered":"http:\/\/iimcb.e-kei.pl\/corefacility\/?page_id=225"},"modified":"2025-03-17T09:30:20","modified_gmt":"2025-03-17T09:30:20","slug":"spr","status":"publish","type":"page","link":"https:\/\/core-facility.iimcb.gov.pl\/spr\/","title":{"rendered":"SPR"},"content":{"rendered":"\t\t
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S<\/u>urface P<\/span>lasmon R<\/u>esonance<\/strong><\/span><\/h2>\n
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Room 612<\/strong><\/span><\/p>\n

Contact: K. Skowronek<\/span><\/a><\/strong><\/span><\/p>\n

Access: collaboration\/contracted research, experienced users (internal)<\/strong><\/span><\/p>\n<\/div>\n

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Biacore S200 (GE Healthcare), 20<\/strong>General description<\/span><\/strong><\/span><\/p>\n

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This technique is used for detailed kinetic characterization of bi-molecular interactions. One of interacting partners (interactants) is immobilized on sensor surface (ligand<\/em><\/strong>) while the other one (analyte<\/em><\/strong>) is injected with a buffer. By measuring SPR angle, changes of refractive index in ~200 nm layer on the sensor surface are recorded in a real time (sensorgram<\/em><\/strong>). This technique does not require labelling of interactants nor impose any limitations on buffer.\u00a0 Series of sensorgrams obtained at different analyte concentrations are used in global fitting to obtain koff<\/sub> and kon <\/sub>kinetic constants of an interaction. The method is relatively fast (one injection usually takes 10-20 minutes) and consumes minute amounts of interactants (~0.5 ml of ligand and analyte solutions at concentrations ~ 5 x expected KD<\/sub>).<\/span><\/p>\n

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Applications<\/span><\/strong><\/span><\/p>\n

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