{"id":1103,"date":"2021-04-22T11:23:35","date_gmt":"2021-04-22T11:23:35","guid":{"rendered":"http:\/\/iimcb.e-kei.pl\/corefacility\/?page_id=1103"},"modified":"2026-01-20T17:53:02","modified_gmt":"2026-01-20T17:53:02","slug":"cd-2","status":"publish","type":"page","link":"https:\/\/core-facility.iimcb.gov.pl\/cd-2\/","title":{"rendered":"CD"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"1103\" class=\"elementor elementor-1103\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-ee4a6c2 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"ee4a6c2\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-a002760\" data-id=\"a002760\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-9f70787 elementor-widget elementor-widget-text-editor\" data-id=\"9f70787\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p style=\"text-align: center;\"><span style=\"color: #000000; font-size: 14pt;\"><strong><u>C<\/u><\/strong><strong>ircular <u>D<\/u>ichroism (CD)<\/strong><\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-fd3b8a5 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"fd3b8a5\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-298d876\" data-id=\"298d876\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-dbb7382 elementor-widget elementor-widget-image\" data-id=\"dbb7382\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"603\" height=\"247\" src=\"https:\/\/core-facility.iimcb.gov.pl\/wp-content\/uploads\/2021\/04\/CD.jpg\" class=\"attachment-large size-large wp-image-1088\" alt=\"CD Jasco\" srcset=\"https:\/\/core-facility.iimcb.gov.pl\/wp-content\/uploads\/2021\/04\/CD.jpg 603w, https:\/\/core-facility.iimcb.gov.pl\/wp-content\/uploads\/2021\/04\/CD-600x246.jpg 600w\" sizes=\"(max-width: 603px) 100vw, 603px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-68931c4 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"68931c4\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-6489bcc\" data-id=\"6489bcc\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-dfcfc8f elementor-widget elementor-widget-text-editor\" data-id=\"dfcfc8f\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p style=\"text-align: center;\"><span style=\"color: #000000; font-size: 14pt;\"><strong>Jasco J-810<\/strong><\/span><br \/><span style=\"color: #000000; font-size: 14pt;\"><strong>Room 612<\/strong><\/span><br \/><span style=\"color: #000000; font-size: 14pt;\"><strong>Contacts: <a href=\"https:\/\/core-facility.iimcb.gov.pl\/krzysztof-skowronek\/\">K. Skowronek<\/a><a href=\"https:\/\/core-facility.iimcb.gov.pl\/roman-szczepanowski2\/\">, Roman <span style=\"color: #3366ff;\">Szczepanowski<\/span><\/a><span style=\"color: #3366ff;\">,<\/span> <a href=\"https:\/\/core-facility.iimcb.gov.pl\/matylda\/\"><span style=\"color: #3366ff;\">Matylda Wac\u0142awska<\/span><\/a><\/strong><\/span><br \/><span style=\"color: #000000; font-size: 14pt;\"><strong>Access: collaboration\/contracted research, trained users<\/strong><\/span><span style=\"color: #000000;\"><strong>\u00a0<\/strong><\/span><\/p><p style=\"text-align: left;\"><span style=\"color: #008000; font-size: 14pt;\"><strong>General description<\/strong><\/span><\/p><p style=\"text-align: left;\"><span style=\"color: #000000;\">CD measures difference in absorption of of right- and left-circularly polarized light by a sample. It equipped with rectangular cuvette holder with temperature controlled by Peltier element. Cuvettes of pathlength range 0.1 \u2013 10 mm are available.<\/span><\/p><p style=\"text-align: left;\"><span style=\"color: #008000; font-size: 14pt;\"><strong>Applications<\/strong><\/span><\/p><ul><li style=\"text-align: left;\"><span style=\"color: #000000;\"><strong>Protein secondary structure assessment<\/strong>. Protein CD spectra in far UV (180 \u2013 250 nm) can be used to asses content of secondary structures by comparison to external libraries of CD spectra for proteins with known secondary structure contents.<\/span><\/li><li style=\"text-align: left;\"><span style=\"color: #000000;\"><strong>Proteins and nucleic acids stability<\/strong>. Changes in CD spectra induced by chemical denaturants or temperature increase allows studies on structure destabilization. The same method can be applied for nucleic acids (but no reference spectra libraries exist).<\/span><\/li><li style=\"text-align: left;\"><span style=\"color: #000000;\"><strong>Structural changes induced by ligand binding<\/strong>. Comparison of ligand-free and ligand-bound protein (or nucleic acid) can be used to infer information about conformational changes induced by ligand binding.<\/span><\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-dae071e elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"dae071e\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-71c6023\" data-id=\"71c6023\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-0aad414 elementor-widget elementor-widget-image\" data-id=\"0aad414\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"634\" height=\"309\" src=\"https:\/\/core-facility.iimcb.gov.pl\/wp-content\/uploads\/2021\/04\/CD1.jpg\" class=\"attachment-large size-large wp-image-1089\" alt=\"CD spectra\" srcset=\"https:\/\/core-facility.iimcb.gov.pl\/wp-content\/uploads\/2021\/04\/CD1.jpg 634w, https:\/\/core-facility.iimcb.gov.pl\/wp-content\/uploads\/2021\/04\/CD1-600x292.jpg 600w\" sizes=\"(max-width: 634px) 100vw, 634px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-b3c7e27 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"b3c7e27\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-c8babcd\" data-id=\"c8babcd\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-4d50202 elementor-widget elementor-widget-text-editor\" data-id=\"4d50202\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"color: #000000;\">Protein analysis. <strong>A<\/strong>. Overlay of CD spectra of proteins with different secondary structure content (BA Wallace, 2019). <strong>B<\/strong> Overlay of BSA and lysozyme spectra.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Circular Dichroism (CD) Jasco J-810Room 612Contacts: K. Skowronek, Roman Szczepanowski, Matylda Wac\u0142awskaAccess: collaboration\/contracted research, trained users\u00a0 General description CD measures difference in absorption of of right- and left-circularly polarized light by a sample. It equipped with rectangular cuvette holder with temperature controlled by Peltier element. Cuvettes of pathlength range 0.1 \u2013 10 mm are available. [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"elementor_header_footer","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"class_list":["post-1103","page","type-page","status-publish","hentry","entry"],"_links":{"self":[{"href":"https:\/\/core-facility.iimcb.gov.pl\/wp-json\/wp\/v2\/pages\/1103","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/core-facility.iimcb.gov.pl\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/core-facility.iimcb.gov.pl\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/core-facility.iimcb.gov.pl\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/core-facility.iimcb.gov.pl\/wp-json\/wp\/v2\/comments?post=1103"}],"version-history":[{"count":28,"href":"https:\/\/core-facility.iimcb.gov.pl\/wp-json\/wp\/v2\/pages\/1103\/revisions"}],"predecessor-version":[{"id":2802,"href":"https:\/\/core-facility.iimcb.gov.pl\/wp-json\/wp\/v2\/pages\/1103\/revisions\/2802"}],"wp:attachment":[{"href":"https:\/\/core-facility.iimcb.gov.pl\/wp-json\/wp\/v2\/media?parent=1103"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}