{"id":1100,"date":"2021-04-22T11:23:03","date_gmt":"2021-04-22T11:23:03","guid":{"rendered":"http:\/\/iimcb.e-kei.pl\/corefacility\/?page_id=1100"},"modified":"2025-03-17T09:29:45","modified_gmt":"2025-03-17T09:29:45","slug":"ftir","status":"publish","type":"page","link":"https:\/\/core-facility.iimcb.gov.pl\/ftir\/","title":{"rendered":"FTIR"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"1100\" class=\"elementor elementor-1100\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-0ae2979 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"0ae2979\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-67e49b5\" data-id=\"67e49b5\" 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2\"><\/w:LsdException><br \/>\n  <w:LsdException Locked=\"false\" Priority=\"66\" Name=\"Medium List 2 Accent 2\"><\/w:LsdException><br \/>\n  <w:LsdException Locked=\"false\" Priority=\"67\" Name=\"Medium Grid 1 Accent 2\"><\/w:LsdException><br \/>\n  <w:LsdException Locked=\"false\" Priority=\"68\" Name=\"Medium Grid 2 Accent 2\"><\/w:LsdException><br \/>\n  <w:LsdException Locked=\"false\" Priority=\"69\" Name=\"Medium Grid 3 Accent 2\"><\/w:LsdException><br \/>\n  <w:LsdException Locked=\"false\" Priority=\"70\" Name=\"Dark List Accent 2\"><\/w:LsdException><br \/>\n  <w:LsdException Locked=\"false\" Priority=\"71\" Name=\"Colorful Shading Accent 2\"><\/w:LsdException><br \/>\n  <w:LsdException Locked=\"false\" Priority=\"72\" Name=\"Colorful List Accent 2\"><\/w:LsdException><br \/>\n  <w:LsdException Locked=\"false\" Priority=\"73\" Name=\"Colorful Grid Accent 2\"><\/w:LsdException><br \/>\n  <w:LsdException Locked=\"false\" Priority=\"60\" Name=\"Light Shading Accent 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6\"><\/w:LsdException><br \/>\n  <w:LsdException Locked=\"false\" Priority=\"64\" Name=\"Medium Shading 2 Accent 6\"><\/w:LsdException><br \/>\n  <w:LsdException Locked=\"false\" Priority=\"65\" Name=\"Medium List 1 Accent 6\"><\/w:LsdException><br \/>\n  <w:LsdException Locked=\"false\" Priority=\"66\" Name=\"Medium List 2 Accent 6\"><\/w:LsdException><br \/>\n  <w:LsdException Locked=\"false\" Priority=\"67\" Name=\"Medium Grid 1 Accent 6\"><\/w:LsdException><br \/>\n  <w:LsdException Locked=\"false\" Priority=\"68\" Name=\"Medium Grid 2 Accent 6\"><\/w:LsdException><br \/>\n  <w:LsdException Locked=\"false\" Priority=\"69\" Name=\"Medium Grid 3 Accent 6\"><\/w:LsdException><br \/>\n  <w:LsdException Locked=\"false\" Priority=\"70\" Name=\"Dark List Accent 6\"><\/w:LsdException><br \/>\n  <w:LsdException Locked=\"false\" Priority=\"71\" Name=\"Colorful Shading Accent 6\"><\/w:LsdException><br \/>\n  <w:LsdException Locked=\"false\" Priority=\"72\" Name=\"Colorful List Accent 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Name=\"Grid Table 6 Colorful Accent 1\"><\/w:LsdException><br \/>\n  <w:LsdException Locked=\"false\" Priority=\"52\" Name=\"Grid Table 7 Colorful Accent 1\"><\/w:LsdException><br \/>\n  <w:LsdException Locked=\"false\" Priority=\"46\" Name=\"Grid Table 1 Light Accent 2\"><\/w:LsdException><br \/>\n  <w:LsdException Locked=\"false\" Priority=\"47\" Name=\"Grid Table 2 Accent 2\"><\/w:LsdException><br \/>\n  <w:LsdException Locked=\"false\" Priority=\"48\" Name=\"Grid Table 3 Accent 2\"><\/w:LsdException><br \/>\n  <w:LsdException Locked=\"false\" Priority=\"49\" Name=\"Grid Table 4 Accent 2\"><\/w:LsdException><br \/>\n  <w:LsdException Locked=\"false\" Priority=\"50\" Name=\"Grid Table 5 Dark Accent 2\"><\/w:LsdException><br \/>\n  <w:LsdException Locked=\"false\" Priority=\"51\" Name=\"Grid Table 6 Colorful Accent 2\"><\/w:LsdException><br \/>\n  <w:LsdException Locked=\"false\" Priority=\"52\" Name=\"Grid Table 7 Colorful Accent 2\"><\/w:LsdException><br \/>\n  <w:LsdException Locked=\"false\" Priority=\"46\" Name=\"Grid Table 1 Light Accent 3\"><\/w:LsdException><br \/>\n  <w:LsdException Locked=\"false\" Priority=\"47\" Name=\"Grid Table 2 Accent 3\"><\/w:LsdException><br \/>\n  <w:LsdException Locked=\"false\" Priority=\"48\" Name=\"Grid Table 3 Accent 3\"><\/w:LsdException><br \/>\n  <w:LsdException Locked=\"false\" Priority=\"49\" Name=\"Grid Table 4 Accent 3\"><\/w:LsdException><br \/>\n  <w:LsdException Locked=\"false\" Priority=\"50\" Name=\"Grid Table 5 Dark Accent 3\"><\/w:LsdException><br \/>\n  <w:LsdException Locked=\"false\" Priority=\"51\" Name=\"Grid Table 6 Colorful Accent 3\"><\/w:LsdException><br \/>\n  <w:LsdException Locked=\"false\" Priority=\"52\" Name=\"Grid Table 7 Colorful Accent 3\"><\/w:LsdException><br \/>\n  <w:LsdException Locked=\"false\" Priority=\"46\" Name=\"Grid Table 1 Light Accent 4\"><\/w:LsdException><br \/>\n  <w:LsdException Locked=\"false\" Priority=\"47\" Name=\"Grid Table 2 Accent 4\"><\/w:LsdException><br \/>\n  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\/>\n\tmso-style-noshow:yes;<br \/>\n\tmso-style-priority:99;<br \/>\n\tmso-style-parent:\"\";<br \/>\n\tmso-padding-alt:0cm 5.4pt 0cm 5.4pt;<br \/>\n\tmso-para-margin-top:0cm;<br \/>\n\tmso-para-margin-right:0cm;<br \/>\n\tmso-para-margin-bottom:8.0pt;<br \/>\n\tmso-para-margin-left:0cm;<br \/>\n\tline-height:107%;<br \/>\n\tmso-pagination:widow-orphan;<br \/>\n\tfont-size:11.0pt;<br \/>\n\tfont-family:\"Calibri\",sans-serif;<br \/>\n\tmso-ascii-font-family:Calibri;<br \/>\n\tmso-ascii-theme-font:minor-latin;<br \/>\n\tmso-hansi-font-family:Calibri;<br \/>\n\tmso-hansi-theme-font:minor-latin;<br \/>\n\tmso-bidi-font-family:\"Times New Roman\";<br \/>\n\tmso-bidi-theme-font:minor-bidi;}<br \/>\n<\/style>\n<p><![endif]--><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-cb35309 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"cb35309\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-3f3dfc9\" data-id=\"3f3dfc9\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-af8108b elementor-widget elementor-widget-image\" data-id=\"af8108b\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"464\" height=\"379\" src=\"https:\/\/core-facility.iimcb.gov.pl\/wp-content\/uploads\/2021\/04\/Tensor.jpg\" class=\"attachment-large size-large 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data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><!-- [if gte mso 9]><xml><br \/> <o:OfficeDocumentSettings><br \/>  <o:AllowPNG><\/o:AllowPNG><br \/> <\/o:OfficeDocumentSettings><br \/><\/xml><![endif]--><!-- [if gte mso 9]><xml><br \/> <w:WordDocument><br \/>  <w:View>Normal<\/w:View><br \/>  <w:Zoom>0<\/w:Zoom><br \/>  <w:TrackMoves><\/w:TrackMoves><br \/>  <w:TrackFormatting><\/w:TrackFormatting><br \/>  <w:PunctuationKerning><\/w:PunctuationKerning><br \/>  <w:ValidateAgainstSchemas><\/w:ValidateAgainstSchemas><br \/>  <w:SaveIfXMLInvalid>false<\/w:SaveIfXMLInvalid><br \/>  <w:IgnoreMixedContent>false<\/w:IgnoreMixedContent><br \/>  <w:AlwaysShowPlaceholderText>false<\/w:AlwaysShowPlaceholderText><br \/>  <w:DoNotPromoteQF><\/w:DoNotPromoteQF><br \/>  <w:LidThemeOther>EN-US<\/w:LidThemeOther><br \/>  <w:LidThemeAsian>X-NONE<\/w:LidThemeAsian><br \/>  <w:LidThemeComplexScript>X-NONE<\/w:LidThemeComplexScript><br \/> 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Priority=\"52\" Name=\"List Table 7 Colorful Accent 6\"><\/w:LsdException><br \/> <\/w:LatentStyles><br \/><\/xml><![endif]--><!-- [if gte mso 10]><\/p>\n<style>\n \/* Style Definitions *\/<br \/> table.MsoNormalTable<br \/>\t{mso-style-name:\"Table Normal\";<br \/>\tmso-tstyle-rowband-size:0;<br \/>\tmso-tstyle-colband-size:0;<br \/>\tmso-style-noshow:yes;<br \/>\tmso-style-priority:99;<br \/>\tmso-style-parent:\"\";<br \/>\tmso-padding-alt:0cm 5.4pt 0cm 5.4pt;<br \/>\tmso-para-margin-top:0cm;<br \/>\tmso-para-margin-right:0cm;<br \/>\tmso-para-margin-bottom:8.0pt;<br \/>\tmso-para-margin-left:0cm;<br \/>\tline-height:107%;<br \/>\tmso-pagination:widow-orphan;<br \/>\tfont-size:11.0pt;<br \/>\tfont-family:\"Calibri\",sans-serif;<br \/>\tmso-ascii-font-family:Calibri;<br \/>\tmso-ascii-theme-font:minor-latin;<br \/>\tmso-hansi-font-family:Calibri;<br \/>\tmso-hansi-theme-font:minor-latin;<br \/>\tmso-bidi-font-family:\"Times New Roman\";<br \/>\tmso-bidi-theme-font:minor-bidi;}<br \/><\/style>\n<p><![endif]--><\/p><p style=\"margin-bottom: 2.0pt; text-align: center; line-height: normal;\" align=\"center\"><span style=\"color: #000000;\"><b style=\"mso-bidi-font-weight: normal;\"><span style=\"font-size: 12.0pt;\">TENSOR 27 with AquaSpec and Bio-ATRII cells (Bruker)<\/span><\/b><\/span><\/p><p style=\"margin-bottom: 2.0pt; text-align: center; line-height: normal;\" align=\"center\"><span style=\"color: #000000;\"><b style=\"mso-bidi-font-weight: normal;\"><span style=\"font-size: 12.0pt;\">Room 612<\/span><\/b><\/span><\/p><p style=\"margin-bottom: 2.0pt; text-align: center; line-height: normal;\" align=\"center\"><b style=\"mso-bidi-font-weight: normal;\"><span style=\"font-size: 12.0pt;\"><span style=\"color: #000000;\">Contact:<\/span> <a href=\"https:\/\/core-facility.iimcb.gov.pl\/krzysztof-skowronek\/\" target=\"_blank\" rel=\"noopener\">K. Skowronek<\/a><\/span><\/b><\/p><p style=\"margin-bottom: 2.0pt; text-align: center; line-height: normal;\" align=\"center\"><span style=\"color: #000000;\"><b style=\"mso-bidi-font-weight: normal;\"><span style=\"font-size: 12.0pt;\">Access: collaboration\/contracted research, trained users<\/span><\/b><\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-3eb95ff elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"3eb95ff\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-f3720c6\" data-id=\"f3720c6\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div 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Accent 6\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"50\" Name=\"List Table 5 Dark Accent 6\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"51\" Name=\"List Table 6 Colorful Accent 6\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"52\" Name=\"List Table 7 Colorful Accent 6\"><\/w:LsdException><br \/> <\/w:LatentStyles><br \/><\/xml><![endif]--><!-- [if gte mso 10]><\/p>\n<style>\n \/* Style Definitions *\/<br \/> table.MsoNormalTable<br \/>\t{mso-style-name:\"Table Normal\";<br \/>\tmso-tstyle-rowband-size:0;<br \/>\tmso-tstyle-colband-size:0;<br \/>\tmso-style-noshow:yes;<br \/>\tmso-style-priority:99;<br \/>\tmso-style-parent:\"\";<br \/>\tmso-padding-alt:0cm 5.4pt 0cm 5.4pt;<br \/>\tmso-para-margin-top:0cm;<br \/>\tmso-para-margin-right:0cm;<br \/>\tmso-para-margin-bottom:8.0pt;<br \/>\tmso-para-margin-left:0cm;<br \/>\tline-height:107%;<br \/>\tmso-pagination:widow-orphan;<br \/>\tfont-size:11.0pt;<br \/>\tfont-family:\"Calibri\",sans-serif;<br \/>\tmso-ascii-font-family:Calibri;<br \/>\tmso-ascii-theme-font:minor-latin;<br \/>\tmso-hansi-font-family:Calibri;<br \/>\tmso-hansi-theme-font:minor-latin;<br \/>\tmso-bidi-font-family:\"Times New Roman\";<br \/>\tmso-bidi-theme-font:minor-bidi;}<br \/><\/style>\n<p><![endif]--><\/p><p><span style=\"color: #339966;\"><b style=\"mso-bidi-font-weight: normal;\"><span style=\"font-size: 14pt;\">General description<\/span><\/b><\/span><\/p><p class=\"MsoNormal\" style=\"margin-bottom: 2.0pt; text-align: justify; line-height: normal;\"><span style=\"color: #000000;\"><span style=\"font-size: 12.0pt;\">Spectrometer is dedicated to FTIR analysis of biological (aqueous) samples using two dedicated measurement cells: AquaSpec \u2013 transmittance cell with optical pathlength of ~ 7 <\/span><span style=\"font-size: 12.0pt; mso-bidi-font-family: Calibri; mso-bidi-theme-font: minor-latin;\">\u00b5<\/span><span style=\"font-size: 12.0pt;\">m and BioATRII, Attenuated Total Reflection cell. Measurements are performed in precisely regulated temperature 4 \u2013 98 \u00b0C. Dedicated OPUS 7.0 software is used for data collection and analysis. Automatic measurements at temperatures ramps are possible.<\/span><\/span><\/p><p class=\"MsoNormal\" style=\"margin-bottom: 2.0pt; text-align: justify; line-height: normal;\"><span style=\"font-size: 12pt; color: #339966;\">\u00a0<\/span><\/p><p class=\"MsoNormal\" style=\"margin-bottom: 2.0pt; text-align: justify; line-height: normal;\"><span style=\"color: #339966;\"><b style=\"mso-bidi-font-weight: normal;\"><span style=\"font-size: 14pt;\">Applications<\/span><\/b><\/span><b style=\"mso-bidi-font-weight: normal;\"><\/b><!-- [if gte mso 9]><xml><br \/> <o:OfficeDocumentSettings><br \/>  <o:AllowPNG><\/o:AllowPNG><br \/> <\/o:OfficeDocumentSettings><br \/><\/xml><![endif]--><!-- [if gte mso 9]><xml><br \/> <w:WordDocument><br \/>  <w:View>Normal<\/w:View><br \/>  <w:Zoom>0<\/w:Zoom><br \/>  <w:TrackMoves><\/w:TrackMoves><br \/>  <w:TrackFormatting><\/w:TrackFormatting><br \/>  <w:PunctuationKerning><\/w:PunctuationKerning><br \/>  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\/>\tmso-para-margin-bottom:8.0pt;<br \/>\tmso-para-margin-left:0cm;<br \/>\tline-height:107%;<br \/>\tmso-pagination:widow-orphan;<br \/>\tfont-size:11.0pt;<br \/>\tfont-family:\"Calibri\",sans-serif;<br \/>\tmso-ascii-font-family:Calibri;<br \/>\tmso-ascii-theme-font:minor-latin;<br \/>\tmso-hansi-font-family:Calibri;<br \/>\tmso-hansi-theme-font:minor-latin;<br \/>\tmso-bidi-font-family:\"Times New Roman\";<br \/>\tmso-bidi-theme-font:minor-bidi;}<br \/><\/style>\n<p><![endif]--><\/p><ul style=\"list-style-type: disc;\"><li class=\"MsoListParagraphCxSpFirst\" style=\"text-align: justify; text-indent: -18pt; line-height: normal;\"><span style=\"color: #000000;\"><span style=\"font-size: 12.0pt; font-family: Wingdings; mso-fareast-font-family: Wingdings; mso-bidi-font-family: Wingdings;\"><span style=\"mso-list: Ignore;\">\u00a0\u00a0\u00a0 <\/span><\/span><b style=\"mso-bidi-font-weight: normal;\"><span style=\"font-size: 12.0pt;\">Protein secondary structure analysis<\/span><\/b><span style=\"font-size: 12.0pt;\">. By analysis of amide I band (1600 -1800 cm<sup>-1<\/sup>) shape in FTIR spectrum, that corresponds to C=O stretching vibration within peptide bonds it is possible to measure secondary structure content of protein in a sample.\u00a0 <\/span><\/span><\/li><li class=\"MsoListParagraphCxSpFirst\" style=\"text-align: justify; text-indent: -18pt; line-height: normal;\"><span style=\"color: #000000;\"><span style=\"font-size: 12.0pt; font-family: Wingdings; mso-fareast-font-family: Wingdings; mso-bidi-font-family: Wingdings;\"><span style=\"mso-list: Ignore;\"><span style=\"font: 7.0pt 'Times New Roman';\">\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 <\/span><\/span><\/span><b style=\"mso-bidi-font-weight: normal;\"><span style=\"font-size: 12.0pt;\">Protein stability analysis. <\/span><\/b><span style=\"font-size: 12.0pt;\">Spectra are recorded in increasing temperature and therefore changes in protein secondary structure induced by heating can be assessed.<\/span><\/span><\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-37a70c7 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"37a70c7\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-9d3860a\" data-id=\"9d3860a\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-2223a8c elementor-widget elementor-widget-image\" data-id=\"2223a8c\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"625\" height=\"771\" src=\"https:\/\/core-facility.iimcb.gov.pl\/wp-content\/uploads\/2021\/04\/FTIR1.jpg\" class=\"attachment-large size-large wp-image-1090\" alt=\"\" srcset=\"https:\/\/core-facility.iimcb.gov.pl\/wp-content\/uploads\/2021\/04\/FTIR1.jpg 625w, https:\/\/core-facility.iimcb.gov.pl\/wp-content\/uploads\/2021\/04\/FTIR1-486x600.jpg 486w\" sizes=\"(max-width: 625px) 100vw, 625px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-180ccef elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"180ccef\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container 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UnhideWhenUsed=\"true\" Name=\"Table List 6\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" SemiHidden=\"true\" UnhideWhenUsed=\"true\" Name=\"Table List 7\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" SemiHidden=\"true\" UnhideWhenUsed=\"true\" Name=\"Table List 8\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" SemiHidden=\"true\" UnhideWhenUsed=\"true\" Name=\"Table 3D effects 1\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" SemiHidden=\"true\" UnhideWhenUsed=\"true\" Name=\"Table 3D effects 2\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" SemiHidden=\"true\" UnhideWhenUsed=\"true\" Name=\"Table 3D effects 3\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" SemiHidden=\"true\" UnhideWhenUsed=\"true\" Name=\"Table Contemporary\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" SemiHidden=\"true\" UnhideWhenUsed=\"true\" Name=\"Table Elegant\"><\/w:LsdException><br \/>  <w:LsdException 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Grid Accent 1\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"63\" Name=\"Medium Shading 1 Accent 1\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"64\" Name=\"Medium Shading 2 Accent 1\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"65\" Name=\"Medium List 1 Accent 1\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" SemiHidden=\"true\" Name=\"Revision\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"34\" QFormat=\"true\" Name=\"List Paragraph\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"29\" QFormat=\"true\" Name=\"Quote\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"30\" QFormat=\"true\" Name=\"Intense Quote\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"66\" Name=\"Medium List 2 Accent 1\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"67\" Name=\"Medium Grid 1 Accent 1\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"68\" Name=\"Medium Grid 2 Accent 1\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"69\" Name=\"Medium Grid 3 Accent 1\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"70\" Name=\"Dark List Accent 1\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"71\" Name=\"Colorful Shading Accent 1\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"72\" Name=\"Colorful List Accent 1\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"73\" Name=\"Colorful Grid Accent 1\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"60\" Name=\"Light Shading Accent 2\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"61\" Name=\"Light List Accent 2\"><\/w:LsdException><br \/>  <w:LsdException Locked=\"false\" Priority=\"62\" Name=\"Light Grid Accent 2\"><\/w:LsdException><br \/>  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\/>\tmso-para-margin-top:0cm;<br \/>\tmso-para-margin-right:0cm;<br \/>\tmso-para-margin-bottom:8.0pt;<br \/>\tmso-para-margin-left:0cm;<br \/>\tline-height:107%;<br \/>\tmso-pagination:widow-orphan;<br \/>\tfont-size:11.0pt;<br \/>\tfont-family:\"Calibri\",sans-serif;<br \/>\tmso-ascii-font-family:Calibri;<br \/>\tmso-ascii-theme-font:minor-latin;<br \/>\tmso-hansi-font-family:Calibri;<br \/>\tmso-hansi-theme-font:minor-latin;<br \/>\tmso-bidi-font-family:\"Times New Roman\";<br \/>\tmso-bidi-theme-font:minor-bidi;}<br \/><\/style>\n<p><![endif]--><\/p><p><span style=\"font-family: georgia, palatino, serif; color: #000000;\"><span style=\"font-size: 10pt;\">Analysis of single amino acid substitutions in MLH1 protein. <\/span><b style=\"mso-bidi-font-weight: normal;\"><span style=\"font-size: 10pt;\">A<\/span><\/b><span style=\"font-size: 10pt;\">. 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Poleszak)<\/span><\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-62689a3 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"62689a3\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-81db755\" data-id=\"81db755\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-0a3cbb4 elementor-widget elementor-widget-text-editor\" data-id=\"0a3cbb4\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><!-- [if gte mso 9]><xml><br \/> <o:OfficeDocumentSettings><br \/>  <o:AllowPNG><\/o:AllowPNG><br \/> <\/o:OfficeDocumentSettings><br \/><\/xml><![endif]--><!-- [if gte mso 9]><xml><br \/> <w:WordDocument><br \/>  <w:View>Normal<\/w:View><br \/>  <w:Zoom>0<\/w:Zoom><br \/>  <w:TrackMoves><\/w:TrackMoves><br \/>  <w:TrackFormatting><\/w:TrackFormatting><br \/>  <w:PunctuationKerning><\/w:PunctuationKerning><br \/>  <w:ValidateAgainstSchemas><\/w:ValidateAgainstSchemas><br \/>  <w:SaveIfXMLInvalid>false<\/w:SaveIfXMLInvalid><br \/>  <w:IgnoreMixedContent>false<\/w:IgnoreMixedContent><br \/>  <w:AlwaysShowPlaceholderText>false<\/w:AlwaysShowPlaceholderText><br \/>  <w:DoNotPromoteQF><\/w:DoNotPromoteQF><br \/>  <w:LidThemeOther>EN-US<\/w:LidThemeOther><br \/>  <w:LidThemeAsian>X-NONE<\/w:LidThemeAsian><br \/>  <w:LidThemeComplexScript>X-NONE<\/w:LidThemeComplexScript><br \/>  <w:Compatibility><br \/>   <w:BreakWrappedTables><\/w:BreakWrappedTables><br \/>   <w:SnapToGridInCell><\/w:SnapToGridInCell><br \/>   <w:WrapTextWithPunct><\/w:WrapTextWithPunct><br \/>   <w:UseAsianBreakRules><\/w:UseAsianBreakRules><br \/>   <w:DontGrowAutofit><\/w:DontGrowAutofit><br \/>   <w:SplitPgBreakAndParaMark><\/w:SplitPgBreakAndParaMark><br \/>   <w:EnableOpenTypeKerning><\/w:EnableOpenTypeKerning><br \/>   <w:DontFlipMirrorIndents><\/w:DontFlipMirrorIndents><br \/>   <w:OverrideTableStyleHps><\/w:OverrideTableStyleHps><br \/>  <\/w:Compatibility><br \/>  <m:mathPr><br \/>   <m:mathFont m:val=\"Cambria Math\"><\/m:mathFont><br \/>   <m:brkBin m:val=\"before\"><\/m:brkBin><br \/>   <m:brkBinSub m:val=\"--\"><\/m:brkBinSub><br \/>   <m:smallFrac m:val=\"off\"><\/m:smallFrac><br \/>   <m:dispDef><\/m:dispDef><br \/>   <m:lMargin m:val=\"0\"><\/m:lMargin><br \/>   <m:rMargin m:val=\"0\"><\/m:rMargin><br \/>   <m:defJc m:val=\"centerGroup\"><\/m:defJc><br \/>   <m:wrapIndent m:val=\"1440\"><\/m:wrapIndent><br \/>   <m:intLim m:val=\"subSup\"><\/m:intLim><br \/>   <m:naryLim m:val=\"undOvr\"><\/m:naryLim><br \/>  <\/m:mathPr><\/w:WordDocument><br \/><\/xml><![endif]--><\/p><p><span style=\"color: #339966;\"><b style=\"mso-bidi-font-weight: normal;\"><span style=\"font-size: 14pt;\">Sample requirements<\/span><\/b><\/span><\/p><p style=\"margin-bottom: 2.0pt; text-align: justify; line-height: normal;\"><span style=\"color: #000000;\"><span style=\"font-size: 12.0pt;\">20 <\/span><span style=\"font-size: 12.0pt; mso-bidi-font-family: Calibri; mso-bidi-theme-font: minor-latin;\">\u00b5<\/span><span style=\"font-size: 12.0pt;\">l and ~50 <\/span><span style=\"font-size: 12.0pt; mso-bidi-font-family: Calibri; mso-bidi-theme-font: minor-latin;\">\u00b5<\/span><span style=\"font-size: 12.0pt;\">l of sample is required for BioATRII and for AquaSpec respectively. The protein concentration should be in 1 \u2013 20 mg\/ml range. <u>Exactly matched buffer (preferably buffer after dialysis, filtrate or fractions preceding peak if sample is obtained directly form chromatography) is absolutely necessary for proper measurement of the background spectrum<\/u>.<\/span><\/span><\/p><p style=\"margin-bottom: 2.0pt; text-align: justify; line-height: normal;\"><span style=\"color: #000000;\"><b style=\"mso-bidi-font-weight: normal;\"><span style=\"font-size: 12.0pt;\">\u00a0<\/span><\/b><\/span><\/p><p style=\"margin-bottom: 2.0pt; text-align: justify; line-height: normal;\"><span style=\"color: #339966;\"><b style=\"mso-bidi-font-weight: normal;\"><span style=\"font-size: 14pt;\">Further reading<\/span><\/b><\/span><\/p><ol><li><span style=\"font-family: georgia, palatino, serif; font-size: 12pt;\"><span style=\"color: #000000;\"><b style=\"mso-bidi-font-weight: normal;\"><span style=\"line-height: 107%;\">Bruker Application Note AN # 404 <\/span><\/b><\/span><span style=\"color: #339966;\"><b style=\"mso-bidi-font-weight: normal;\"><span style=\"line-height: 107%;\">(<a href=\"https:\/\/www.bruker.com\/fileadmin\/user_upload\/8-PDF-Docs\/OpticalSpectrospcopy\/FT-IR\/CONFOCHECK\/AN\/AN404_Study_protein_conformation_EN.pdf\">https:\/\/www.bruker.com\/fileadmin\/user_upload\/8-PDF-Docs\/OpticalSpectrospcopy\/FT-IR\/CONFOCHECK\/AN\/AN404_Study_protein_conformation_EN.pdf<\/a>)<\/span><\/b><\/span><\/span><span style=\"font-family: georgia, palatino, serif; font-size: 12pt;\"><span style=\"color: #339966;\"><b style=\"mso-bidi-font-weight: normal;\"><span style=\"line-height: 107%;\"><br \/><\/span><\/b><\/span><\/span><\/li><li><span style=\"font-family: georgia, palatino, serif; font-size: 12pt; color: #000000;\"><b style=\"mso-bidi-font-weight: normal;\"><span style=\"line-height: 107%;\"><span style=\"line-height: 107%;\">Fabian H and M\u00e4ntele HW. Infrared Spectroscopy of Proteins. doi: <\/span><\/span><\/b><b style=\"mso-bidi-font-weight: normal;\"><span style=\"line-height: 107%;\"><span class=\"MsoHyperlink\"><b><span style=\"line-height: 107%; background: white none repeat scroll 0% 0%;\"><a style=\"color: #000000;\" href=\"https:\/\/doi.org\/10.1002\/0470027320.s8201\">10.1002\/0470027320.s8201<\/a><\/span><\/b><\/span><\/span><\/b><\/span><br \/><b style=\"mso-bidi-font-weight: normal;\"><b><\/b><\/b><\/li><li><span style=\"font-family: georgia, palatino, serif; font-size: 12pt;\"><a href=\"https:\/\/www.chem.uwec.edu\/chem455_s05\/pages\/Manuals\/FTIR_of_proteins.pdf\">https:\/\/www.chem.uwec.edu\/chem455_s05\/pages\/Manuals\/FTIR_of_proteins.pdf<\/a><\/span><\/li><li><span style=\"font-family: georgia, palatino, serif; font-size: 12pt; color: #000000;\">Carbonaro M, Nucara A. Secondary structure of food proteins by Fourier transform spectroscopy in the mid-infrared region. Amino Acids. (2010) <strong>38<\/strong>(3):679-90. doi: 10.1007\/s00726-009-0274-3. PMID: 19350368.<\/span><\/li><li><span style=\"font-family: georgia, palatino, serif; font-size: 12pt; color: #000000;\">Kong J, Yu S. Fourier transform infrared spectroscopic analysis of protein secondary structures. Acta Biochim Biophys Sin (Shanghai). (2007) <strong>39<\/strong>(8):549-59. PMID: 17687489.<\/span><\/li><\/ol><p style=\"text-align: justify; text-indent: -18.0pt; mso-list: l0 level1 lfo1;\"><span style=\"font-family: georgia, palatino, serif; font-size: 12pt;\"><span style=\"mso-list: Ignore;\">1.<\/span><\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Fourier-Transform Infrared Spectrometer (FTIR) TENSOR 27 with AquaSpec and Bio-ATRII cells (Bruker) Room 612 Contact: K. 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