{"id":1097,"date":"2021-04-22T11:22:12","date_gmt":"2021-04-22T11:22:12","guid":{"rendered":"http:\/\/iimcb.e-kei.pl\/corefacility\/?page_id=1097"},"modified":"2025-03-17T09:30:23","modified_gmt":"2025-03-17T09:30:23","slug":"uplc","status":"publish","type":"page","link":"https:\/\/core-facility.iimcb.gov.pl\/uplc\/","title":{"rendered":"UPLC"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"1097\" class=\"elementor elementor-1097\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-00dbdc6 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"00dbdc6\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-09cc601\" data-id=\"09cc601\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-bcf0dd4 elementor-widget elementor-widget-text-editor\" data-id=\"bcf0dd4\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p style=\"text-align: center;\"><span style=\"font-size: 14pt; color: #000000;\"><strong>Ultra Performance Liquid Chromatography (UPLC)<\/strong><\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-578ef20 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"578ef20\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-e5162be\" data-id=\"e5162be\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-852ebe2 elementor-widget elementor-widget-image\" data-id=\"852ebe2\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"614\" height=\"426\" src=\"https:\/\/core-facility.iimcb.gov.pl\/wp-content\/uploads\/2021\/04\/UPLC1.jpg\" class=\"attachment-large size-large wp-image-1092\" alt=\"UPLC\" srcset=\"https:\/\/core-facility.iimcb.gov.pl\/wp-content\/uploads\/2021\/04\/UPLC1.jpg 614w, https:\/\/core-facility.iimcb.gov.pl\/wp-content\/uploads\/2021\/04\/UPLC1-600x416.jpg 600w\" sizes=\"(max-width: 614px) 100vw, 614px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-fbe9d2b elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"fbe9d2b\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-804ada8\" data-id=\"804ada8\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-2725a29 elementor-widget elementor-widget-text-editor\" data-id=\"2725a29\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p style=\"text-align: center;\"><span style=\"font-size: 14pt; color: #000000;\"><strong>ACQUITY (Waters)<\/strong><\/span><br \/><span style=\"font-size: 14pt; color: #000000;\"><strong>Room 607A<\/strong><\/span><br \/><span style=\"font-size: 14pt; color: #000000;\"><strong>Contact: <a href=\"https:\/\/core-facility.iimcb.gov.pl\/krzysztof-skowronek\/\">K. Skowronek<\/a><\/strong><\/span><br \/><span style=\"font-size: 14pt; color: #000000;\"><strong>Access: collaboration\/contracted research, experienced users<\/strong><\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-bb1e371 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"bb1e371\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-de3af2b\" data-id=\"de3af2b\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-dd5a366 elementor-widget elementor-widget-text-editor\" data-id=\"dd5a366\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"color: #008000;\"><strong>General description<\/strong><\/span><\/p><ul><li><span style=\"color: #000000;\">High-end analytical liquid chromatography system with UV\/VIS and fluorometric detectors.<\/span><\/li><li><span style=\"color: #000000;\">PDA e\u03bb Detector: spectral range 190 \u2013 800 nm (up to 2.0 AU linearity), cell volume 500 nl<\/span><\/li><li><span style=\"color: #000000;\">FLR detector: spectral range 200 \u2013 890 (excitation), 210 \u2013 900 (emission), bandwidth 20 nm, cell volume &lt;2 \u00b5l<\/span><\/li><li><span style=\"color: #000000;\">Solvent system: binary, up to 15 000 psi<\/span><\/li><li><span style=\"color: #000000;\">Autosampler: up to 96 samples, injection volume 3 \u2013 250 \u00b5l<\/span><\/li><li><span style=\"color: #000000;\">Column heaters: 20 &#8211; 90\u00b0C, one for 4 columns up to 15 cm, second for a single column up to 30 cm<\/span><\/li><li><span style=\"color: #000000;\">Selection of Reverse Phase (C-18) and Size Exclusion Chromatography (Aquity UPLC BEH200 \u00c5, resolution range 10 \u2013 450 kDa) columns<\/span><\/li><\/ul><p><span style=\"color: #008000;\"><strong>Applications<\/strong><\/span><\/p><ul><li><span style=\"color: #000000;\">Qualitative analysis of complex mixtures (RP) of absorbing or fluorescent compounds<\/span><\/li><li><span style=\"color: #000000;\">Quantification of a single compound in complex mixtures<\/span><\/li><li><span style=\"color: #000000;\">Quaternary structure analysis of isolated macromolecular complexes<\/span><\/li><li><span style=\"color: #000000;\">Quaternary structure analysis of fluorescent proteins in complex mixtures (including clarified cell lysates)<\/span><\/li><li><span style=\"color: #000000;\">Other applications can be developed<\/span><\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-73562f9 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"73562f9\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-95e1076\" data-id=\"95e1076\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-6a6b75b elementor-widget elementor-widget-image\" data-id=\"6a6b75b\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"629\" height=\"273\" src=\"https:\/\/core-facility.iimcb.gov.pl\/wp-content\/uploads\/2021\/04\/UPLC2.jpg\" class=\"attachment-large size-large wp-image-1093\" alt=\"\" srcset=\"https:\/\/core-facility.iimcb.gov.pl\/wp-content\/uploads\/2021\/04\/UPLC2.jpg 629w, https:\/\/core-facility.iimcb.gov.pl\/wp-content\/uploads\/2021\/04\/UPLC2-600x260.jpg 600w\" sizes=\"(max-width: 629px) 100vw, 629px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-83a60e2 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"83a60e2\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-35f4b97\" data-id=\"35f4b97\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-8511910 elementor-widget elementor-widget-text-editor\" data-id=\"8511910\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"color: #000000;\">Examples of analysis: <strong>A<\/strong>. SEC: protein calibration mix (sizes in kDa are shown above peaks). <strong>B<\/strong>. Reverse phase fractionation of deoxynucleosides mix on C18 column. <strong>C<\/strong>. Calibration for caffeine concentration measurements in complex mixtures<\/span><\/p><p>\u00a0<\/p><p><span style=\"color: #008000;\"><strong>Sample requirements<\/strong><\/span><\/p><p><span style=\"color: #000000;\">Samples should be clarified, preferably by filtration through 0.2 \u00b5m filter. If filtration is not possible use supernatant after through centrifugation (at least 5 min. at &gt; 10.000 g). Volumes 5 -250 \u00b5l can be injected on RP columns and 5 \u2013 50 \u00b5l on SEC column.<\/span><\/p><p>\u00a0<\/p><p><span style=\"color: #008000;\"><strong>Further reading<\/strong><\/span><\/p><ol><li><a href=\"https:\/\/www.waters.com\/waters\/keywordSearch.htm?cid=505548&amp;q=UPLC&amp;qTemp=UPLC\">https:\/\/www.waters.com\/waters\/keywordSearch.htm?cid=505548&amp;q=UPLC&amp;qTemp=UPLC<\/a><\/li><li><a href=\"https:\/\/www.waters.com\/waters\/keywordSearch.htm?cid=505548&amp;q=empower+3&amp;filter=searchcontentcategory%7CKLIB&amp;page=1\">https:\/\/www.waters.com\/waters\/keywordSearch.htm?cid=505548&amp;q=empower+3&amp;filter=searchcontentcategory%7CKLIB&amp;page=1<\/a><\/li><\/ol>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Ultra Performance Liquid Chromatography (UPLC) ACQUITY (Waters)Room 607AContact: K. SkowronekAccess: collaboration\/contracted research, experienced users General description High-end analytical liquid chromatography system with UV\/VIS and fluorometric detectors. PDA e\u03bb Detector: spectral range 190 \u2013 800 nm (up to 2.0 AU linearity), cell volume 500 nl FLR detector: spectral range 200 \u2013 890 (excitation), 210 \u2013 900 [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"elementor_header_footer","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"class_list":["post-1097","page","type-page","status-publish","hentry","entry"],"_links":{"self":[{"href":"https:\/\/core-facility.iimcb.gov.pl\/wp-json\/wp\/v2\/pages\/1097","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/core-facility.iimcb.gov.pl\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/core-facility.iimcb.gov.pl\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/core-facility.iimcb.gov.pl\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/core-facility.iimcb.gov.pl\/wp-json\/wp\/v2\/comments?post=1097"}],"version-history":[{"count":12,"href":"https:\/\/core-facility.iimcb.gov.pl\/wp-json\/wp\/v2\/pages\/1097\/revisions"}],"predecessor-version":[{"id":2050,"href":"https:\/\/core-facility.iimcb.gov.pl\/wp-json\/wp\/v2\/pages\/1097\/revisions\/2050"}],"wp:attachment":[{"href":"https:\/\/core-facility.iimcb.gov.pl\/wp-json\/wp\/v2\/media?parent=1097"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}