2200 TapeStation System
(Agilent)
Room: 609
Contact: Katarzyna Misztal
Access: short training required, no reservation needed
General Description
The Agilent 2200 TapeStation is fast and automated system for electrophoresis. The instrument provieds analysis of size, guality and quantity of DNA and RNA samples. The samples are resolved in precasted gel tapes called ScreenTapes. The results are obtained in one minute per sample. It is possible to analyze 16 samples per one run (or any other even number of samples up to 16).
ScreenTapes and their parameters:
Molecule | ScreenTape | Size Range | Quantiative Range | Sample Volume |
genomic DNA | Genomic DNA | 200 – 60 000bp | 10 – 100 ng/ul | 1 ul |
DNA | D1000 | 35 -1000 bp | 1 ul | |
DNA | High Sensitivity D1000 | 35 -1000 bp | 10 – 1000 pg/ul | 2 ul |
DNA | D5000 | 100 – 5000 bp | 0.1 – 50 ng/ul | 1 ul |
DNA | High Sensitivity D5000 | 100 – 5000 bp | 10 – 1000 pg/ul | 2 ul |
RNA | RNA | 100 – 6000 bp | 25 – 500 ng/ul | 1 ul |
RNA | High Sensitivity RNA | 100 – 6000 bp | 0.5 – 10 ng/ul | 2 ul |
Genomic DNA, D1000, HS D1000 and HS RNA ScreenTapes are availabe in CF.
Applications
- DNA and RNA sample quality check including DNA and RNA Integrity Number (DIN and RIN) calculation
- Size control for next generation sequencing libraries
Further reading
Agilent 2200 TapeStation System User Manual.pdf
Check the Agilent technology website for more details.